Abstract
ABSTRACTT1N6_22, a short-chain dehydrogenase/reductase family protein, was identified as a positive regulator in Arabidopsis thaliana resistance against Botrytis cinerea and Alternaria brassicae in our preliminary study. In this study, we found that the expression levels of the T1N6_22 gene were induced and up-regulated in A. thaliana ecotype Columbia (Col-0) after B. cinerea and Pseudomonas syringae pv. tomato DC3000 inoculation. Compared with the Col-0 and t1n6_22/T1N6_22 plants, the expression of PAL, PR4, PPO, SOD and CAT genes were down-regulated in the t1n6_22 plants. In Col-0 plants treated with salicylic acid (SA) and the SA analogue benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH), the expression levels of T1N6_22 were significantly enhanced, whereas the expression levels of T1N6_22 were reduced by jasmonic acid treatment. Meanwhile, the t1n6_22 mutant exhibited enhanced resistance, whereas the wild-type Col-0 and complemented plants (t1n6_22/T1N6_22) showed susceptibility to Pst DC3000. After inoculation with B. cinerea and Pst DC3000, the expression levels of defence-related genes PR1, PR3, PR5, NPR1 and PDF1.2 in t1n6_22 were significantly different from those in Col-0 and t1n6_22/T1N6_22 plants. Taken together, the T1N6_22 gene played a negative role in Arabidopsis resistance to Pst DC3000. The T1N6_22 gene may be involved in the regulation of salicylic acid and jasmonic-acid–signalling pathways to affect the resistance of Arabidopsis to B. cinerea and Pst DC3000.
Highlights
Grey mould, caused by the fungus Botrytis cinerea, occurs worldwide and causes significant economic losses every year
Different defence mechanisms are involved in the response against different pathogens: the salicylic acid (SA)-dependent response is deployed against the biotrophic pathogens Pseudomonas syringae or Hyaloperonospora parasitica, whereas the JA/ET response is activated by the necrotrophic pathogens B. cinerea or Alternaria brassicicola [9,10,11]
PR1 (PATHOGENESIS RELATED 1) and NPR1 are commonly used as marker genes that have been found to have a profound impact on the SA-signalling pathway [14,15]
Summary
Grey mould, caused by the fungus Botrytis cinerea, occurs worldwide and causes significant economic losses every year. Many reports indicate that plant defence responses against pathogens are mediated by either salicylic acid (SA), or jasmonate/ethylene (JA/ET) signalling pathway [1,2,3]. Different defence mechanisms are involved in the response against different pathogens: the SA-dependent response is deployed against the biotrophic pathogens Pseudomonas syringae or Hyaloperonospora parasitica, whereas the JA/ET response is activated by the necrotrophic pathogens B. cinerea or Alternaria brassicicola [9,10,11]. These two molecules play important roles in the regulation of signalling networks in induced defence responses. Plant defence responses have been achieved through activation of genes encoding antimicrobial
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