γδT Cells Positively Regulate Contact Sensitivity (CS) Reaction via Modulation of INF-γ, IL-12 and TNF-α Production

Anna Strzępa,Monika Majewska-Szczepanik,Marian Szczepanik

doi:10.3409/fb61_3-4.205

Abstract

The gammadeltaT cells were identified as positive as well as negative regulators of immune responses. They take part in pathogen clearance, modulation of innate and adaptive immunity as well as in healing and tissue maintenance. The course of many pathological conditions such as collagen induced arthritis (CIA), experimental autoimmune encephalomyelitis (EAE) and airway hyperresponsiveness is positively regulated by gammadeltaT cells. It was shown previously that contact sensitivity (CS), an example of antigen-specific cell-mediated immune response, is also positively regulated by gammadeltaT cells. The current work confirmed the regulatory function of gammadeltaT cells in CS response as their depletion with anti-TCRdelta monoclonal antibody and complement significantly decreased adoptive transfer of the CS reaction. In vitro study showed that removal of gammadeltaT cells with magnetic beads significantly decreased the production of the proinflammatory cytokines IFN-gamma, IL-12 and TNF-alpha. Reconstitution of gammadeltaT-depleted cells with gammadeltaT-enriched cells restored cytokine production, proving the reversibility of the investigated process. In summary, gammadeltaT cells positively regulate the CS reaction via modulation of proinflammatory cytokine production.

Highlights

STRZÊPA A., MAJEWSKA-SZCZEPANIK M., SZCZEPANIK M. 2013. ãäT cells positively regulate contact sensitivity (CS) reaction via modulation of INF-ã, IL-12 and TNF-á production
Cytokine production was evaluated in culture supernatants
The immunomostimulatory role of ãäT cells has been previously shown in animal models of collagen induced arthritis (CIA) (ROARK et al 2007), EAE (PONOMAREV et al 2004) and airway hyperresponsiveness (COOK et al 2008). ãäT cells were identified as a cell population that assists áâT CS-effector cells (PTAK et al 1996; SZCZEPANIK et al 1998)

Summary

Introduction

STRZÊPA A., MAJEWSKA-SZCZEPANIK M., SZCZEPANIK M. 2013. ãäT cells positively regulate contact sensitivity (CS) reaction via modulation of INF-ã, IL-12 and TNF-á production. ÃäT cells positively regulate contact sensitivity (CS) reaction via modulation of INF-ã, IL-12 and TNF-á production. It was shown previously that contact sensitivity (CS), an example of antigen-specific cell-mediated immune response, is positively regulated by ãäT cells. It is notable that allergic contact dermatitis resulting from exposure to chemicals in the workplace constitutes about 30% of all occupational diseases (DIEPGEN & WEISSHAAR 2007) This type of response may occur due to long-term exposure of the skin to low molecular weight substances, including heavy metals (e.g. chromium, nickel, cobalt), latex, turpentine, fragrances and preservatives in cosmetics, epoxy resins and their hardeners, as well as some drugs applied in ointments (e.g. Neomycin). Repeated exposure to such compounds elicits migration of previously induced TNP-specific T cells to the site of elicitation, leading to a local inflammatory response in the skin Clinical manifestations of this reaction involve edema, erythema as well as oozed papules, pustules and itching

Methods

Results

Conclusion