T cell subsets and Langerhans cells in the forestomach mucosa of adult sheep and sheep foetuses

Abstract

As part of a study on leukocytes in the ruminant forestomach the occurrence and distribution of T cells and major histocompatibility complex Class II positive (MHC-II +) cells in the forestomach mucosa of sheep were investigated. Samples were taken from the reticulum, atrium ruminis, ventral rumen, dorsal rumen and omasum of five healthy adult ewes and seven nearly fullborne foetuses. Frozen sections were stained with an indirect immunoperoxidase method using monoclonal antibodies against sheep MHC-II, CD4 and CD8 molecules and the sheep γδ T cell receptor. Both MHC-II + cells and T cells were distributed along the basal lamina of the epithelium, with either mainly intraepithelial (CD8 + and γδ + cells) or mainly subepithelial (MHC-II + and CD4 + cells) location. The MHC-II + cells showed dendritic morphology and were interpreted as Langerhans cells. In adult ewes CD4 + T cells comprised the major T cell subset at all sample sites. The number of T cells declined from the atrium ruminis through the ventral rumen to the dorsal rumen, while the number of Langerhans cells showed no marked variation between different sample sites. In foetuses, Langerhans cells showed a relatively high prevalence, while T cells were sparse and showed a more random distribution in the rumen wall. No marked variation between sample sites were observed in the fetal forestomachs. It is concluded that Langerhans cells and T cells are normally present in the forestomach mucosa of sheep, and it is suggested that the Langerhans cells represent a constituent component, while the prevalence and distribution of T cells may be influenced by antigen leakage through the epithelium.