T cell specific and non specific tolerance mechanisms in peanut oil immunotherapy in peanut allergic subjects

Amit Singh,Sue Neale-May,Tessa Hunter,Brittany Weldon,Grace Yu,Kari Nadeau

doi:10.1186/2045-7022-1-s1-o34

Abstract

Rationale Our main focus in the laboratory is to understanding the mechanisms of how oral immunotherapy (OIT) improves outcomes in patients. In this study, we aimed to determine if OIT is specific only to the food allergens administered in OIT or also to other offending allergens (i.e. “bystander effect”). To accomplish our aim, we studied T cell reactivity (i.e. proliferation assays) and T cell specificity (i.e. tetramer assays with peanut peptides) in both effector T cell (CD4+CD25neg) or Teff and regulatory T cell (CD4+CD25hiFoxp3+) or Treg subsets.

Highlights

Rationale Our main focus in the laboratory is to understanding the mechanisms of how oral immunotherapy (OIT) improves outcomes in patients
We aimed to determine if OIT is specific only to the food allergens administered in OIT or to other offending allergens (i.e. “bystander effect”)
We studied T cell reactivity and T cell specificity in both effector T cell (CD4+CD25neg) or Teff and regulatory T cell (CD4+CD25hiFoxp3+) or Treg subsets

Summary

Introduction

Rationale Our main focus in the laboratory is to understanding the mechanisms of how oral immunotherapy (OIT) improves outcomes in patients. We aimed to determine if OIT is specific only to the food allergens administered in OIT or to other offending allergens We studied T cell reactivity (i.e. proliferation assays) and T cell specificity (i.e. tetramer assays with peanut peptides) in both effector T cell (CD4+CD25neg) or Teff and regulatory T cell (CD4+CD25hiFoxp3+) or Treg subsets

Objectives

Methods

Conclusion