Abstract
Several studies have shown the importance of evaluating Recent Thymic Emigrants (RTEs) by quantification of T cell receptor-rearrangement excision circles (TRECs), as a measure of de novo T cell generation during human immunodeficiency virus-1 (HIV-1) infection. To determine whether acute viral infection may have an impact on TRECs, cynomolgus monkeys ( Macaca fascicularis) were infected intrarectally with simian human immunodeficiency virus (SHIV) 89.6P cy11 and the number of signal-joint (sj) TRECs was determined in purified CD4 + and CD8 + populations for up to 28 weeks post-infection. Four weeks after infection, TRECs levels significantly decreased in both CD3 +CD4 + and in CD3 +CD8 + T lymphocytes of infected monkeys, whereas they remained unchanged in uninfected animals. This reduction was followed by a progressive TRECs number recovery in CD3 +CD4 + T lymphocytes that positively correlated with changes in the levels of circulating CD3 +CD4 + T cells. In the CD3 +CD8 + T cell subset, TRECs number remained significantly low and inversely correlated with the increase in the percentages of CD3 +CD8 + T cells. These data suggest that SHIV89.6P cy11 intrarectal infection of cynomolgus monkeys differently affects TRECs content in CD3 +CD4 + and CD3 +CD8 + T cell subsets.
Published Version
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