西达本胺联合苦参碱对皮肤T细胞淋巴瘤细胞系增殖、凋亡的影响及可能的凋亡机制

Abstract

Objective To evaluate the effect of chidamide combined with matrine on proliferation and apoptosis of cutaneous T-cell lymphoma (CTCL) cell lines HH and Hut78, and to explore their apoptotic mechanisms. Methods Both HH and Hut78 cells were treated with 0.4 μmol/L chidamide and 0.6 g/L matrine alone or in combination for 24, 48 and 72 hours, with those treated with dimethyl sulfoxide (DMSO) serving as control groups. MTS assay was performed to detect cellular proliferation rates of HH and Hut78 cells at each time point. After 48-hour treatment, flow cytometry was conducted to detect cell apoptosis, and Western blot analysis to determine expression of apoptosis-related proteins in these cells. Statistical analysis was carried out by using repeated measures analysis of variance, one-way analysis of variance, and least significant difference (LSD) -t test for multiple comparisons. Results Compared with DMSO, chidamide and matrine alone or in combination could inhibit the proliferation of HH and Hut78 cells to different extents (F = 15.88, 558.26, P 0.05) , while the combination group (20.94% ± 0.64%) showed a significantly higher apoptosis rate compared with the control group, matrine group and chidamide group (LSD-t = 7.37, 5.40, 5.69 respectively, all P 0.05) . Conclusion Chidamide in combination with matrine can inhibit proliferation and induce apoptosis of HH and Hut78 cells, likely by regulating the expression of apoptosis-related proteins E-cadherin, NF-κB, p-Bad, Bcl-2 and cleaved caspase-3. Key words: Lymphoma, T-cell, cutaneous; Matrine; Apoptosis; Chidamide; Hut78 cells; HH cells