Abstract
Qi-Jing-Sheng-Bai granule (QJSB) is a newly developed traditional Chinese medicine (TCM) formula. Clinically, it has been used for the treatment of leucopenia. However, its pharmacological mechanism needs more investigation. In this study, we firstly tested the effects of QJSB on leucopenia using mice induced by cyclophosphamide. Our results suggested that QJSB significantly raised the number of peripheral white blood cells, platelets and nucleated bone marrow cells. Additionally, it markedly enhanced the cell viability and promoted the colony formation of bone marrow mononuclear cells. Furthermore, it reversed the serum cytokines IL-6 and G-CSF disorders. Then, using transcriptomics datasets and metabonomic datasets, we integrated transcriptomics-based network pharmacology and metabolomics technologies to investigate the mechanism of action of QJSB. We found that QJSB regulated a series of biological processes such as hematopoietic cell lineage, homeostasis of number of cells, lymphocyte differentiation, metabolic processes (including lipid, amino acid, and nucleotide metabolism), B cell receptor signaling pathway, T cell activation and NOD-like receptor signaling pathway. In a summary, QJSB has protective effects to leucopenia in mice probably through accelerating cell proliferation and differentiation, regulating metabolism response pathways and modulating immunologic function at a system level.
Highlights
Chemotherapy has been widely used for the treatment of cancers
We got the similar results when the leucopenia model animals were treated by Qi-Jing-Sheng-Bai granule (QJSB) for 2 weeks (Figures 1E,F)
The detail parameters of pathways and GO terms are shown in Tables 1, 2. These results indicate that QJSB may influence these pathways and biological process, increasing peripheral white blood cells (WBC) and platelets in leucopenia model mice
Summary
Chemotherapy has been widely used for the treatment of cancers. Chemotherapy usually induces many adverse effects, including hematologic toxicity and neurotoxicity (Magge and DeAngelis, 2015; Ratti and Tomasello, 2015; Zhou et al, 2018). Hematologic toxicity, like leucopenia, is a very common adverse reaction that can delay the subsequent therapy, induce the risk of cancer metastasis, and even lead to life-threatening events (Xu et al, 2011; Cui et al, 2015). Qi-Jing-Sheng-Bai Granule on Leucopenia in Mice related agents have clinical efficacy. Traditional Chinese medicine (TCM) formulae have been widely used in China for the treatment of leucopenia (Liu et al, 2014). It is believed that multiple components contained in the formulae could hit multiple targets and exert synergistic effects (Jiang, 2005)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
