Systemic treatment with resveratrol reduces the progression of experimental periodontitis and arthritis in rats.

Mônica G Corrêa,Paula Rodrigues Pires,Fabiano Ribeiro Cirano,Marcio Z Casati,Marcelo Henrique Napimoga,Renato Corrêa Viana Casarin,Fernanda Vieira Ribeiro,Suzana Peres Pimentel

doi:10.1371/journal.pone.0204414

Mônica G Corrêa, Paula Rodrigues Pires + Show 6 more

Open Access

https://doi.org/10.1371/journal.pone.0204414

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Journal: PloS one	Publication Date: Oct 3, 2018
Citations: 38	License type: CC BY 4.0

Affiliation: Universidade Paulista, Faculdade São Leopoldo Mandic

Abstract

Rheumatoid arthritis and periodontitis are chronic inflammatory diseases which has been closely associated due to the nature of immune-inflammatory imbalance response. Resveratrol is a naturall product with biological proprieties that may promote immunomodulatory effects on host response. This study investigated resveratrol continuous administration effect on experimental periodontitis and arthritis progression in rats. Thirty-five rats were assigned to the following groups: 1—experimental arthritis + experimental periodontitis + placebo (RA+EP +PL) (n = 12); 2 –RA+EP+ ibuprofen (RA+PE+IB) (n = 11); 3—RA+EP+ resveratrol (RA+PE+RSV) (n = 11). After euthanasia, the specimens were processed for morphometric analysis of bone loss, and the gingival tissue surrounding the first molar was collected for quantification of inflammatory markers using a Luminex/MAGpix assay and anti-citrullinated protein antibody (ACCPA) levels were measured by ELISA assay. Serum level of rheumatoid factor (RF) was measured by ELISA assay. Paw edema was analyzed using a plethysmometer. Higher bone loss was observed in PL group, when compared to IB and RSV groups. RSV group presented higher IL-4 concentration than PL and IB groups. Resveratrol reduced RF serum levels and both IB and RSV decreased ACCPA gingival levels. Besides, paw swelling level was significantly lower in IB and RSV groups in the 21th day and only in RSV group in the 28th day. Histological analyzes showed smooth articular surface and higher width of the subchondral cortical in RSV group. Resveratrol showed modulatory effect and seems to reduce the inflammatory signs of arthritis and articular damage throughout the time.

Highlights

Rheumatoid arthritis is a chronic inflammatory autoimmune disease with a prevalence of 0.5% to 1.0% in adults in industrialized countries that results in bone and cartilage loss [1]
In face of the above evidence, this study investigated the effect of continuous use of resveratrol systemically administered on the progression of experimental periodontitis and arthritis, on the local and serum cytokine levels and on the clinical and articular feature of RA condition
The link between rheumatoid arthritis and periodontitis and the bidirectional pathway of their development has been reported by important clinical studies [6,7,8,9,10,11, 45,46,47] and systematic reviews [12, 48,49,50]

Summary

Introduction

Rheumatoid arthritis is a chronic inflammatory autoimmune disease with a prevalence of 0.5% to 1.0% in adults in industrialized countries that results in bone and cartilage loss [1]. The authors observed increased risk of periodontitis in the presence of RA compared to healthy controls (relative risk: 1.13; 95% CI: 1.04, 1.23) with significantly worst clinical periodontal parameters in those with RA [12] These findings are confirmed in several animal studies, which show more bone loss and higher levels of inflammatory cytokines in the presence of RA [13,14,15,16]. In face of the above evidence, this study investigated the effect of continuous use of resveratrol systemically administered on the progression of experimental periodontitis and arthritis, on the local and serum cytokine levels and on the clinical and articular feature of RA condition

Material and methods

Result

Findings

Discussion