Abstract
BackgroundThe purpose of this study was to investigate mediators of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis (CNPA), a locally, destructive process of the lung due to invasion by Aspergillus species.MethodsMeasurements of selected biomarkers in 10 patients with CNPA and 19 healthy, matched controls were performed with enzyme-linked immunosorbent assay (ELISA) and multiplex methodology. The gene expressions of relevant biomarkers were analyzed with real-time quantitative RT-PCR.ResultsIncreased concentrations of circulating mediators of inflammation interleukin (IL)-6, IL-8, RANTES, TNF-α, ICAM-1 and mediators involved in endothelial activation and thrombosis (vWF, TF and PAI-1) were observed in patients with CNPA. The concentration of the anti-inflammatory cytokine IL-10 was increased both in plasma and in PBMC in the patient population. The gene expression of CD40L was decreased in PBMC from the patient group, accompanied by decreased concentrations of soluble (s) CD40L in the circulation.ConclusionsThe proinflammatory response against Aspergillus may be counteracted by reduced CD40L and sCD40L, as well as increased IL-10, which may compromise the immune response against Aspergillus in patients with CNPA.
Highlights
The purpose of this study was to investigate mediators of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis (CNPA), a locally, destructive process of the lung due to invasion by Aspergillus species
Chronic necrotizing pulmonary aspergillosis (CNPA) is a locally destructive process of the lung due to semi invasive infection by Aspergillus species often accompanied by development of a fungus ball
Markers of inflammation and endothelial activation Several significant differences were observed between CNPA patients (n = 10) and healthy controls (n = 19) with respect to inflammatory and anti-inflammatory mediators as well as markers of endothelial activation
Summary
The purpose of this study was to investigate mediators of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis (CNPA), a locally, destructive process of the lung due to invasion by Aspergillus species. The ubiquitous mould Aspergillus fumigatus causes a variety of clinical syndromes ranging from allergic disease in immunocompetent individuals to invasive pulmonary aspergillosis in severely immunocompromised patients [1,2]. Chronic necrotizing pulmonary aspergillosis (CNPA) is a locally destructive process of the lung due to semi invasive infection by Aspergillus species often accompanied by development of a fungus ball (aspergilloma). CNPA which is a relatively uncommon form of Aspergillus infection, typically occurs as a complication of preexisting chronic lung diseases such as measured selected biomarkers involved in inflammation and thrombosis in 10 patients with CNPA and in 19 sexand age-matched healthy individuals
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