Abstract
BackgroundRestoration of oxygen carrying capacity (OCC) is essential to adequately treat hemorrhagic shock. Multifunctional resuscitation fluids (MRFs) containing oxygen carrier (OC) molecules are being developed to improve OCC. However, adverse vasoactivity of previous generations of OCs has limited MRF development. Newer, third generation PFCs have garnered interest as potential OCs for use in MRFs. Here, as part of an evaluation as a potential OC for use in a MRF, we tested a third generation PFC for systemic and microvascular vasoactivity in a rodent model.MethodsMale Sprague‐Dawley rats (250‐350 grams) were randomly assigned to receive four sequential intravenous infusions (1.5 mL/kg each) of either PFC (Oxycyte®; n=10) or 0.9% NaCl (control; n=10). At the conclusion of each study, phenylephrine (PE; 1 mg/kg) was infused to confirm physiological intactness of the animal. Heart rate (HR) and mean arterial pressure (MAP) were continuously monitored. Vasoactivity was assessed by intravital microscopy of spinotrapezius arcade arterioles. Statistical comparisons were made by ANOVA. Results reported as mean ± SEM.ResultsNo observed differences were seen in MAP, HR, or microvascular arteriole diameters between groups at baseline or throughout the study (Table 1). In both PFC and control groups, PE constricted arterioles by 50% (p<0.001) and elevated MAP 2‐fold (p<0.001). PFC (n=10) Control (n=10) Baseline Final Dose PE Baseline Final Dose PE MAP (mmHg) 94 ± 4 99 ± 5 198 ± 3 95 ± 2 90 ± 4 199 ± 5 HR (bpm) 362 ± 6 344 ± 36 326 ± 10 364 ± 9 359 ± 10 314 ± 11 Arteriole Diameter (µm) 58 ± 4 58 ± 4 26 ± 4 57 ± 2 62 ± 4 24 ± 4 Funding: DoD Hemorrhage and Resuscitation Research and Development Program (WUN G1018)ConclusionAlthough these data demonstrate no adverse systemic or microcirculatory vasoactive effects, further evaluation of the third generation PFC in a clinically relevant hemorrhage model is warranted.
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