Abstract
Polymeric test surfaces of P(l-d,l)LA and of a P(l-d,l)LA:PLGA blend decorated with 25 nanopillar covered fields, were used to investigate differences in growth of bone marrow stem cells (BMSC) and osteosarcoma cells (Saos-2). The fields were populated with pillars (ca. 900nm tall, 200nm×200nm area) separated systematically from each other with 1–10μm gaps. Saos-2 cells populated fields decorated with pillars 1μm apart but they avoided pillar-free surfaces. In contrast, BMSCs avoided fields with interpillar distances <2μm. Both BMSCs and Saos-2 cells aligned in the direction of the shorter distance when at least one of the interpillar distances was greater than 1.5μm. Coating the P(l-d,l)LA surfaces with cell adhesive protein fibronectin enabled the BMSC to populate fields with high pillar density which they had avoided when uncoated. Decreasing the stiffness of the film surface by using a blend of (P(l-d,l)LA and PLGA) made them more acceptable for attachment by the BMSC cells.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
