Systematic Transmission Electron Microscopy‐Based Identification and 3D Reconstruction of Cellular Degradation Machinery (Adv. Biology 6/2023)

Abstract

3D Reconstruction Macroautophagy is a dynamic process that regulates cellular mechanisms. It can be difficult to identify machinery and quantify them. In article 2200221, Antentor O. Hinton Jr. and co-workers demonstrate the use of transmission electron microscopy, immunogold labeling, immunofluorescence, and 3D reconstruction serial block face-scanning electron microscopy to identify and quantify lysosomes, autophagosomes, and lipid droplets under drug response conditions and experimental knockouts.