Abstract
High-speed countercurrent chromatography (HSCCC) and silica gel column chromatography were used to separate and purify alkaloids from Chinese herbal medicine Euchresta tubulosa Dunn. The purpose of this study is to provide a system mode for rapid separation of alkaloids from natural products. In the experiment, the eluent of silica gel column chromatography was screened by thin layer chromatography (TLC) to obtain four components with different polarity. Then, the two-phase solvent systems of different components were selected and purified by HSCCC. Four alkaloids with relatively high content were obtained by this mode successfully, including matrine (28 mg), oxymatrine (32 mg), N-formyl cytisine (24 mg), and cytisine (58 mg). The purity was higher than 91% by high performance liquid chromatography–ultraviolet (HPLC-UV) and their chemical structures were identified by nuclear magnetic resonance (NMR) and electron ionization mass spectrometry (EI-MS). The results showed that the combination of HSCCC and silica gel column chromatography could make alkaloids from natural products separate systematically.
Highlights
Euchresta tubulosa Dunn. (Leguminosae, Euchresta) is the evergreen shrub mainly distributed in Sichuan, Chongqing, Hunan and Hubei [1]
Alkaloids in congeneric plants (Euchresta) were separated only by a single method such as silica gel column chromatography, thin-layer chromatography and sephadex LH-20 [12,13], which have obvious defects, and it is difficult to achieve the purpose of systematic separation and purification
Four alkaloids with relatively high content including matrine, oxymatrine, N-formyl cytisine and cytisine in Euchresta tubulosa Dunn., one of the most important chinese medicinal herbs, were separated by silica gel column chromatography combined with High-speed countercurrent chromatography (HSCCC)
Summary
Euchresta tubulosa Dunn. (Leguminosae, Euchresta) is the evergreen shrub mainly distributed in Sichuan, Chongqing, Hunan and Hubei [1]. (Leguminosae, Euchresta) is the evergreen shrub mainly distributed in Sichuan, Chongqing, Hunan and Hubei [1]. It is known as Odougen, Hudoulian, Dougan, Opium Seven in china. In Japan, it was used to treat ulcers and arrhythmias [2]. It was often used to treat sore throat and abdominal pain by Tujia minority. Alkaloids in congeneric plants (Euchresta) were separated only by a single method such as silica gel column chromatography, thin-layer chromatography and sephadex LH-20 [12,13], which have obvious defects, and it is difficult to achieve the purpose of systematic separation and purification
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