Systematic review of fNIRS studies reveals inconsistent chromophore data reporting practices.

Abstract

Functional near-infrared spectroscopy (fNIRS) is unique among neuroimaging techniques in its ability to estimate changes in both oxyhemoglobin (HbO) and deoxyhemoglobin (HbR). However, fNIRS research has applied various data reporting practices based on these chromophores as measures of neural activation. To quantify the variability of fNIRS chromophore data reporting practices and to explore recent data reporting trends in the literature. We reviewed 660 fNIRS papers from 2015, 2018, and 2021 to extract information on fNIRS chromophore data reporting practices. Our review revealed five general practices for reporting fNIRS chromophores: (1)HbO only, (2)HbR only, (3)HbO and HbR, (4)correlation-based signal improvement, and (5)either the total (HbT) or difference (HbDiff) in concentration between chromophores. The field was primarily divided between reporting HbO only and reporting HbO and HbR. However, reporting one chromophore (HbO) was consistently observed as the most popular data reporting practice for each year reviewed. Our results highlight the high heterogeneity of chromophore data reporting in fNIRS research. We discuss its potential implications for study comparison efforts and interpretation of results. Most importantly, our review demonstrates the need for a standard chromophore reporting practice to improve scientific transparency and, ultimately, to better understand how neural events relate to cognitive phenomena.