Abstract
A systematic method for determination of 6 kinds of pesticides (thiabendazole, benomyl, imazalil, diphenyl, o-phenylphenol and 2, 4-D) in citrus fruits was developed. The pesticides were extracted from the citrus fruits with acetone and the extract was concentrated. After addition of ethyl acetate to the concentrated solution, 2, 4-D and the basic pesticides (thiabendazole, benomyl and imazalil) were partitioned into an alkaline aqueous layer (pH 9) and then an acid layer (0.1N HCl), respectively.The basic pesticides were re-extracted from the acid layer with ethyl acetate at pH 10, and the extract was subjected to high performance liquid chromatography (HPLC) with either UV detection or fluorescence detection. The ethyl acetate layer was concentrated for the determination of diphenyl and o-phenylphenol, and subjected to HPLC with fluorescence detection. 2, 4-D was re-extracted from the alkaline aqueous layer with ethyl ether and methylated, and the resulting derivative was determined by gas chromatography-mass spectrometry with selected ion monitoring after clean-up.The recoveries of 6 pesticides added to citrus fruits at 0.2-1.0μg/g were in the range of 80.5-97.3%. The detection limits were 0.02-0.2μg/g.
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