Abstract
Background: The role of hyaluronan-mediated motility receptor (HMMR) in colorectal cancer (CRC) remains unclear. The present study aimed to explore the association of HMMR with the development and prognosis of CRC using sequence datasets, clinical tissues, blood samples, and cell lines. Methods: CRC datasets were downloaded from TCGA and GEO databases. Forty CRC tissue samples, 120 CRC blood samples, and 100 healthy controls were collected. Four CRC cell lines (HCT116, HT-29, LoVo, and SW480) and one normal human colon mucosal epithelial cell line (NCM460) were cultured. RT-qPCR was used to determine the expression of HMMR in the tissues and cell lines. ELISA was used to measure HMMR levels in the blood samples. Results: The expression of HMMR was significantly increased in CRC tissues than in corresponding adjacent tissues based on TCGA and GEO datasets, and clinical CRC tissues. No associations were found between the expression of HMMR and the TNM stage or other clinical parameters. The expression of HMMR varied in different CRC cell lines. The blood levels of HMMR tended to be higher in patients with CRC than in healthy controls. TCGA and GEO datasets showed inconsistent results regarding the association of HMMR expression with the survival of patients with CRC. Conclusion: The expression of HMMR is increased in CRC tissues but not in the blood. The expression of HMMR is independent of CRC development and has no prognostic significance in patients with CRC.
Highlights
Colorectal cancer (CRC) is one of the most common malignancies worldwide, according to the GLOBOCAN 2020 reports (Sung et al, 2021)
We first analyzed the expression of hyaluronan-mediated motility receptor (HMMR) mRNA in gastrointestinal cancers in The Cancer Genome Atlas (TCGA), including colon cancer (COAD), rectal cancer (READ), liver cancer (LIHC), gastric cancer (STAD), and esophageal cancer (ESCA) datasets, and found that HMMR mRNA was significantly overexpressed in gastrointestinal cancer tissues than in normal tissues (Figure 1A, p < 0.05)
We analyzed the expression of HMMR mRNA in GSE21815, GSE31279, GSE44076, GSE32323, and GSE113513 datasets and found that the expression of HMMR was significantly increased in the CRC tissues than in normal tissues (Figures 1B–F, p < 0.05)
Summary
Colorectal cancer (CRC) is one of the most common malignancies worldwide, according to the GLOBOCAN 2020 reports (Sung et al, 2021). Despite advances in surgical techniques, and adjuvant and neoadjuvant therapies, the prognosis of patients with CRC at a later stage remains poor. Several molecules are associated with the development and progression of CRC, and some of them can serve as indicators of CRC diagnosis, treatment monitoring, and prognosis (Di Nicolantonio et al, 2021). Other molecules still need to be explored. The role of hyaluronan-mediated motility receptor (HMMR) in colorectal cancer (CRC) remains unclear. The present study aimed to explore the association of HMMR with the development and prognosis of CRC using sequence datasets, clinical tissues, blood samples, and cell lines
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