Systematic Analysis of Kelch Repeat F-box (KFB) Protein Gene Family and Identification of Phenolic Acid Regulation Members in Salvia miltiorrhiza Bunge.

Haizheng Yu,Bingxue Zhang,Lijun Liang,Bingcong Xing,Zongsuo Liang,Mengdan Jiang

doi:10.3390/genes11050557

Abstract

S. miltiorrhiza is a well-known Chinese herb for the clinical treatment of cardiovascular and cerebrovascular diseases. Tanshinones and phenolic acids are the major secondary metabolites and significant pharmacological constituents of this plant. Kelch repeat F-box (KFB) proteins play important roles in plant secondary metabolism, but their regulation mechanism in S. miltiorrhiza has not been characterized. In this study, we systematically characterized the S. miltiorrhiza KFB gene family. In total, 31 SmKFB genes were isolated from S. miltiorrhiza. Phylogenetic analysis of those SmKFBs indicated that 31 SmKFBs can be divided into four groups. Thereinto, five SmKFBs (SmKFB1, 2, 3, 5, and 28) shared high homology with other plant KFBs which have been described to be regulators of secondary metabolism. The expression profile of SmKFBs under methyl jasmonate (MeJA) treatment deciphered that six SmKFBs (SmKFB1, 2, 5, 6, 11, and 15) were significantly downregulated, and two SmKFBs (SmKFB22 and 31) were significantly upregulated. Tissue-specific expression analysis found that four SmKFBs (SmKFB4, 11, 16, and 17) were expressed preferentially in aerial tissues, while two SmKFBs (SmKFB5, 25) were predominantly expressed in roots. Through a systematic analysis, we speculated that SmKFB1, 2, and 5 are potentially involved in phenolic acids biosynthesis.

Highlights

S. miltiorrhiza, which belongs to the family Labiate, is a well-known Chinese herb
S. miltiorrhiza SmKFB gene, AtKFB sequences were used as queries to blast against the current assembly of the S. miltiorrhiza genome
Among 49 putative SmKFBs, F-box or Kelch motif were absent in 18 genes

Summary

Introduction

S. miltiorrhiza, which belongs to the family Labiate, is a well-known Chinese herb. The dried roots and rhizomes of this plant are known as Danshen in The Pharmacopoeia of The People’s Republic of China, and they are widely used in the clinical treatment of cardiovascular and cerebrovascular diseases in China and, to a lesser degree, in Japan, The United States, and other European countries [1].Phenolic acids and tanshinones are major pharmaceutical components and secondary metabolites in S. miltiorrhiza. S. miltiorrhiza, which belongs to the family Labiate, is a well-known Chinese herb. The dried roots and rhizomes of this plant are known as Danshen in The Pharmacopoeia of The People’s Republic of China, and they are widely used in the clinical treatment of cardiovascular and cerebrovascular diseases in China and, to a lesser degree, in Japan, The United States, and other European countries [1]. Phenolic acids and tanshinones are major pharmaceutical components and secondary metabolites in S. miltiorrhiza. In China, many pharmaceutical dosage forms of Danshen are commercially available, such as dripping pills, tablets, injectables, oral liquids, and granules. Among all the available dosage forms, the Fufang Danshen dripping pill and Fufang Danshen tablets

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