Syringotoxin pore formation and inactivation in human red blood cell and model bilayer lipid membranes

Abstract

The effect of syringotoxin (ST), a member of the cyclic lipodepsipeptides family (CLPs) produced by Pseudomonas syringae pv. syringae on the membrane permeability of human red blood cells (RBCs) and model bilayer lipid membranes (BLMs) was studied and compared to that of two recently investigated CLPs, syringomycin E (SRE) and syringopeptin 22A (SP22A) [Biochim. Biophys. Acta 1466 (2000) 79 and Bioelectrochemistry 52 (2000) 161]. The permeability-increasing effect of ST on RBCs was the least among the three CLPs. A time-dependent ST pore inactivation was observed on RBCs at 20 and 37 °C but not at 8 °C. From the kinetic model worked out parameters as permeability coefficient of RBC membrane for 86Rb + and pores mean lifetime were calculated. A shorter pores mean lifetime was calculated at 37 °C then at 20 °C, which gave us an explanation for the unusual slower rate of tracer efflux measured at 37 °C then that at 20 °C. The results obtained on BLM showed that the pore inactivation was due to a decrease in the number of pores but not to a change of their dwell time or conductance.