Syrah Grape Skin Residues Has Potential as Source of Antioxidant and Anti-Microbial Bioactive Compounds.

Roberta Barreto De Andrade,Gabriele De Abreu Barreto,Renata Quartieri Nascimento,Marcelo Andrés Umsza-Guez,Luiz Claudio Corrêa,Bruna Aparecida Souza Machado,Ingrid Lessa Leal,Ederlan De Souza Ferreira,Pedro Paulo Lordelo Guimarães Tavares

doi:10.3390/biology10121262

Roberta Barreto De Andrade, Gabriele De Abreu Barreto + Show 7 more

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https://doi.org/10.3390/biology10121262

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Abstract

Simple SummaryThe aim of this study was to verify the influence of different extraction parameters (temperature and ultrasound time) of bioactive compounds from the skin of the Syrah variety of grape. Among the extracts obtained, those exposed to 20 min of sonication had the best results in terms of flavonoid content, antioxidant potential and phenolic profile. The temperature of 60 °C provided the most relevant results for the content of total phenolics, stilbenes, flavonols and phenolic acids, however, the association of this temperature with the use of ultrasound showed lower results as a source of antioxidant and antimicrobial bioactive compounds.In this study, we evaluated the effects of ultrasound-assisted extraction (UAE) under different time-temperature conditions on the content of bioactive compounds, antioxidant and antimicrobial activities of Syrah grape skin residue. The application of UAE showed a positive effect on the extraction of total flavonoids, and a negative effect on total polyphenols. The temperature of 40 °C and 60 °C without the UAE caused an increase of 260% and 287% of the total polyphenols, respectively. Nineteen individually bioactive compounds were quantified. The anthocyanin concentration (malvidin-3,5-di-O-glucoside 118.8–324.5 mg/100 g) showed high variation, to a lesser extent for phenolic acids, flavonoids, flavonols, procyanidins and stilbenes due to the UAE process. The Syrah grape skin residue has a high concentration of total phenolic compounds of 196–733.7 mg·GAE/100 g and a total flavonoid content of 9.8–40.0 mg·QE/100 g. The results of free radical scavenging activity (16.0–48.7 mg/100 mL, as EC50) and its inhibition of microbial growth (0.16 mg/mL, as EC50 for S. aureus, and 0.04 mg/mL, as EC50 for E. coli) by grape skin extract (UAE 40:20) indicate high antioxidant and antibacterial activity. It was concluded that the use of ultrasound needs further analysis for its application in this context, as it has shown deleterious effects on some compounds of interest. Syrah grape skin residue has potential as a source of bioactive antioxidants, antimicrobial activity and for use as a functional food ingredient.

Highlights

In recent years, the valorization of agro-industrial by-products has become evident, due to the extensive information available on their valuable contents of bioactive compounds that promote many beneficial effects on human health, such as reducing the risk of developing chronic disease, e.g., cancer, neurodegenerative and cardiovascular diseases [1,2].In this sense, it includes viticulture, considered one of the most important agricultural activities around the world [3], which, from the solid winemaking by-products or grape marc, presents a high amount of residual polyphenols [4], and a low-cost source of natural antioxidants that have been used by pharmaceutical, cosmetic and food industries [5]
Different studies that aim at higher yields of phenolic compounds from plant matrices correlate the oscillation of results to the application of process variables, such as use time and temperature variation [32,33,34]
Data regarding the content of total phenolic compounds are often associated with the capacity of antioxidant properties [5,35]

Summary

Introduction

The valorization of agro-industrial by-products has become evident, due to the extensive information available on their valuable contents of bioactive compounds that promote many beneficial effects on human health, such as reducing the risk of developing chronic disease, e.g., cancer, neurodegenerative and cardiovascular diseases [1,2] In this sense, it includes viticulture, considered one of the most important agricultural activities around the world [3], which, from the solid winemaking by-products or grape marc, presents a high amount of residual polyphenols [4], and a low-cost source of natural antioxidants that have been used by pharmaceutical, cosmetic and food industries [5]. Several authors have reported the antimicrobial activity of different grape skin extracts for different types of microorganisms, such as on Gram-positive and Gram-negative bacteria and fungi, pathogenic or not [12,13,14]

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