Abstract
Substrates for neuron culture and implantation are required to be both biocompatible and display surface compositions that support cell attachment, growth, differentiation, and neural activity. Laminin, a naturally occurring extracellular matrix protein is the most widely used substrate for neuron culture and fulfills some of these requirements, however, it is expensive, unstable (compared to synthetic materials), and prone to batch-to-batch variation. This study uses a high-throughput polymer screening approach to identify synthetic polymers that supports the in vitro culture of primary mouse cerebellar neurons. This allows the identification of materials that enable primary cell attachment with high viability even under "serum-free" conditions, with materials that support both primary cells and neural progenitor cell attachment with high levels of neuronal biomarker expression, while promoting progenitor cell maturation to neurons.
Highlights
This study uses a high-throughput polymer screening approach of substrates, e.g., fibronectin, collagen, to identify synthetic polymers that supports the in vitro culture of primary mouse cerebellar neurons. This allows the identification of materials that enable primary cell attachment with high viability even under “serumfree” conditions, with materials that support both primary cells and neural polylysine, that support in vitro culture and neuronal expansion is limited; with laminin perhaps the most widely used surface coating for in vitro studies.[5]
Damage to the adult central nervous system caused by physical research on neural regeneration as well as to achieve clinical injuries, inflammation, or cancer cannot regenerate on its own.[1] translation, factors that influence neuron growth need to be. Surgical treatments such as tissue transplan- understood, ideally with neurons that are cultured in chemitation and nerve grafting, have been used for the reparation cally defined media to minimize the influence of growth facof damaged regions, but encounter limitations with regard to tors and/or extracellular matrix proteins—which again would appropriate donor sites and shortages of material and are prone cause problematic regulatory issues
neural progenitor cells (NPCs) cultured on these three polymers were analyzed for the expression of Glial fibrillary acidic protein (GFAP)[18] and TUJ1[19] studied using immunohistochemistry, to determine the maturation progression of NPCs into neural cells
Summary
Substrates for neuron culture and implantation are required to be both regeneration.[3] To minimize immunobiocompatible and display surface compositions that support cell attachment, growth, differentiation, and neural activity. De Zeeuw Department of Neuroscience Erasmus MC Rotterdam Rotterdam NL-3015 GE, The Netherlands E-mail: c.dezeeuw@erasmusmc.nl
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