Year-end Sale % OFF 
Abstract
Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antiviral peptides emerge as effective and relatively safe therapeutic molecules. Based on in silico analysis of VP2 protein from IPNV and the RNA-dependent RNA polymerase from ISAV, a set of peptides was designed and were chemically synthesized to block selected key events in their corresponding infectivity processes. The peptides were tested in fish cell lines in vitro, and four were selected for decreasing the viral load: peptide GIM182 for IPNV, and peptides GIM535, GIM538 and GIM539 for ISAV. In vivo tests with the IPNV GIM 182 peptide were carried out using Salmo salar fish, showing a significant decrease of viral load, and proving the safety of the peptide for fish. The results indicate that the use of peptides as antiviral agents in disease control might be a viable alternative to explore in aquaculture.
Highlights
Aquaculture is an important industry in Chile, with at least three species of farmed salmon, Coho salmon (Oncorhynchus kisutch), rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar), cultivated in net pens with the latter being the second most valuable export product of the country, after copper.Fish farming involves great risks at the sanitary level, with the development of pathogens that seriously affect the sustainability of the industry
Primary sequences for the infectious pancreatic necrosis virus (IPNV) polyprotein and the precursor pVP2 protein of the different genogroups (Uniprot accession P05844, Q990P9, Q703G9, Q990P7, Q990P5, Q990P8) have an identity above 80%, and in the case of the cognate birnavirus infectious bursal disease virus (IBDV) identity was above 30% (Uniprot accession P61825)
In recent years significant progress has been made to elucidate the mechanisms of action of antimicrobial peptides (AMPs) and their involvement in disease control in humans [44]; notwithstanding, there are other biological systems in which this approach should be highly beneficial
Summary
Aquaculture is an important industry in Chile, with at least three species of farmed salmon, Coho salmon (Oncorhynchus kisutch), rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar), cultivated in net pens with the latter being the second most valuable export product of the country, after copper.Fish farming involves great risks at the sanitary level, with the development of pathogens that seriously affect the sustainability of the industry. IPNV is a member of the Birnaviridae family and the etiological agent of infectious pancreatic necrosis, one of the main diseases that affect salmonid fish in general; in Chile, it represents the second cause of infectious mortality in fry [1]. It does not currently represent a high risk, survivors of infection become asymptomatic carriers of the virus for life, acting as reservoirs and spreaders of progeny viruses through the water via feces mainly during stress episodes, and as breeders, Pathogens 2020, 9, 600; doi:10.3390/pathogens9080600 www.mdpi.com/journal/pathogens. Some animals that recover from infection become labile which decreases productivity and health performance
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
