Abstract
The authors have constructed a synthetic chromosome arm of the yeast Saccharomyces cerevisiae, laying the groundwork for creating a synthetic eukaryotic genome. A ~90 kb stretch of the right arm of chromosome IX was designed in silico to remove unstable elements such as transposons and to facilitate genome manipulations. The synthetic sequence, which was swapped into the endogenous genomic region, also contains an inducible evolution system, SCRaMbLE (synthetic chromosome rearrangement and modification by loxP-mediated evolution), which triggers combinatorial rearrangements to allow the study of complex phenotypes.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.