Abstract
Laccase immobilization is a promising method for the purification of pre-hydrolysis liquor (PHL) for large-scale applications based on recyclability and reusability. In this work, magnetic supports made of Fe3O4/SiO2 were synthesized by a sol-gel method and coated with silica. Then, glutaraldehyde was used as a linker between the support and laccase for immobilization. The immobilized laccase was evaluated by purifying the PHL from a kraft-based dissolving pulp production process. The results showed that the optimal immobilization conditions were a molar TEOS/Fe3O4 ratio of 0.6, glutaraldehyde concentration of 6%, laccase concentration of 0.4 mL/mg, and time of 3 h, which led to 71% laccase immobilization. The concentration of total sugar in the PHL increased from 71% to 75%, while the lignin content decreased from 15% to 10% after the immobilized laccase treatment. The separation advantage of the magnetic support enhanced the utility of the laccase.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
