Synthesis titanium dioxide nanoparticles doped with silver and Novel antibacterial activity

Abstract

Pure and Ag doped TiO2 nanoparticles were synthesis via a hydrothermal process and have anti-bacterial activity were examined against Escherichia coli, Staphylococcus aureus. The effect of Ag doping on TiO2 to rutile phase transformation was investigated by X-ray diffraction (XRD), Scanning electron microscopy (SEM),(EDX), Fourier transform infrared (FTIR) spectroscopy, and (UV). The average size of the TiO2 was in the range of (12.6)nm, and the doped TiO2+Ag had an average size of (12)nm. The antibacterial activity of the Tio2 and TiO2+Ag were evaluated against E. coli,S. aureus using the release of cellular materials method. The results showed that TiO2+Ag nanoparticles as a novel DNA-mediated antibacterial agent. The TiO2+Ag nanoparticles were observed to destroy the bacterial cells by permeating the bacterial nucleic acid and cytoplasmic membrane, resulting in the loss of cell wall integrity, nucleic acid damage, and increased cell-wall permeability.