Synthesis, spectroscopic characterizations and biological activities of vanadyl(II) folate compound as a new anti-DNA damage and antioxidant agent

Abstract

New oxovanadium(IV) folate [(VO)2(FO)(NH4)2(SO4)2] complex was synthesized by the reaction between vanadyl(II) sulfate and folic acid vitamin B9 drug in an alkaline media. Elemental analysis shows 1:2 ligand to metal ion stoichiometry and the conductance data deduced that oxovanadium(IV) folate complex has non-electrolytic nature. IR spectrum of oxovanadium(IV) complex reveal that folic acid acts as a binuclear chelate via deprotonation of both carboxylic groups. The biological section aimed to test the toxicity of carbofuran orally for male rat and oxidative stress of the sub-lethal (2.4mg/kg b.w, 1/25 LD50) dose on the lipid peroxidation level (LPO), reduced glutathione content (GSH), antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activities of testicular tissue. The protective efficiency of vanadylfolate (50mg/kg b.w) only or combined together carbofuran was tested. The taken of carbofuran orally led to elevation in LPO level by 2.10 fold in comparable with control. The efficient of antioxidant enzymes using of vanadylfolate in combined with carbofuran or its only were decreased by (25.48%, 18.60%, 22.46% and 16.62%) but the GSH level increased by 37.18% in testicular tissue in comparison with controller sample. The DNA damaging of carbofuran and vanadylfolate were assessed using single cell gel electrophoresis (SCGE) data.