Synthesis, physicochemical characterisation and cytotoxic screening of new complexes of cerium, lanthanum and neodymium with Warfarin and Coumachlor sodium salts

Abstract

The complexes of the types Ln(HW) 3• nH 2O and Ln(HC) 3• nH 2O [where Ln = Ce, La, Nd; HW = Warfarin; HC = Coumachlor] have been synthesized by reaction of Warfarin Sodium, resp. Coumachlor Sodium and the appropriate lanthanide nitrates in various stoichiometric ratios. The formation of the complexes have been proved on the basis of elemental analysis, conductivities, IR spectroscopy and 1H-NMR spectroscopy. It is concluded that the lacton- and the keto-carbonyl groups of the ligands are bonded to the metal ion as bidentate ligand. The conductivity measurements show non-electrolytic nature of the complexes. Cytotoxicity determination by MTT-assay shows that the inorganic salts and the complexes with Warfarin did not show any significant activity. The complexes with Coumachlor were found to be more cytotoxic. The most active compound was the complex of Cerium with Coumachlor. All tested complexes showed similar in vitro cytotoxic profiles. This fact is in agreement with the formation of the complexes.