Synthesis, physico-chemical and DNA interaction studies of homo- and hetero-trinuclear complexes

Abstract

Synthesis and characterization of three new trinuclear metal complexes of type Cu 3, Cu 2Zn and Cu 2Ni have been achieved by assembling simple mononuclear complexes, namely 2,2′-bipyridyl 3,4-dihydroxo benzaldehyde copper(II) complex and diethylenetriamine complexes of copper(II), nickel(II) and zinc(II) ions, through the reaction of coordinated ligands. The FAB mass spectra for the complexes show fragmentation pattern in accordance with the molecular formula. The frozen electron paramagnetic resonance (EPR) spectrum of tricopper complex shows two sets of parallel lines with approximately 2:1 ratio. The simulation has been carried out by considering dipolar interaction between the two types of copper ions present in the complex. The trimetallic complexes, Cu 3, Cu 2Ni and Cu 2Zn show strong intercalation type of interaction with Calf thymus DNA in 0.02 mol L −1 of phosphate buffer containing 60 mmol sodium chloride at pH 7.0 at room temperature. The binding constant is found to be in the order Cu 3 < Cu 2Zn < Cu 2Ni. The enhanced binding capability of Cu 2Ni complex is attributed to the increased symmetry in the overall structure of the complex and the pronounced binding character of positively charged Ni(II) ions with the purines.