Abstract
A new ruthenium(II) complex [Ru(Happip)3](ClO4)2 {Happip=2-(4-(β-d-allopyranoside)phenyl)imidazo[4,5-f][1,10]phenanthroline} was synthesized and characterized by elemental analysis, 1H NMR and matrix-assisted laser desorption ionization mass spectrometry. Calf-thymus DNA-binding properties were studied by DNA viscosity measurements and spectroscopic methods of UV-visible (UV-vis) absorption and luminescence titrations, steady-state emission quenching, DNA competitive binding with ethidium bromide and DNA melting experiments, indicating that the complex partially intercalates into the DNA with a large binding constant greater than 106M−1. The pH effects on UV-vis absorption and emission spectra of the complex were studied, demonstrating that the complex acted as an excellent pH-induced “on–off–on” luminescence switch with large on–off intensity ratios of 88 and 50 with one of luminescence on/off switching actions occurring in near-physiological pH region (pKa2=7.33). The DNA photocleaving properties of [Ru(Happip)3]2+ were also studied and compared with those of [Ru(bpy)2(Happip)]2+ and [Ru(bpy)2(Hpip)]2+{bpy=2,2′-bipyridine, Hpip=2-(4-phenyl)imidazo[4,5-f][1,10]phenanthroline}.
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