Synthesis, optimization, and characterization of silver/gold allied bimetallic from Hippeastrum hybridum (L.) and their ex vivo anti‐acetylcholinesterase activity in rat brain

Abstract

Nanotechnology, a modern technique, is apprehensive for small‐size nanoparticle (NP) generation via superficial, straightforward, and pharmaceutically modes. The main aim of the current research was to project the development of a green justifiable method to synthesize Ag/Au bimetallic NPs (BNPs) via Hippeastrum hybridum (HH) extract and then to use these BNPs as rat brain anti‐acetylcholinesterase (anti‐AChE) in ex vivo mode. According to the current results, BNPs exhibited a sharp peak of 542 nm when characterized by ultravioet (UV)–visible spectroscopy; Fourier transform infrared (FT‐IR) analysis exhibited peaks, which confirmed the existence of various phytochemicals in HH plant extract; X‐ray diffraction (XRD) analysis confirmed the crystal BNPs 8.34 nm mean size; scanning electron microscopy (SEM) showed that BNPs had a square shape with 20‐nm nanomeasures calculated sizes; and energy dispersive X‐ray (EDX) analysis confirmed Ag and Au composition, which was reported to be 12% and 38.26%, respectively. Moreover, the anti‐AChE potential of BNPs was also tested to cure Alzheimer's disease (AD). An excellent anti‐AChE activity was exhibited by BNPs in which AChE exhibited 31 ± 0.22% activity at 150‐μg concentration of BNPs with 103 ± 0.42 μg IC50. Statistical analysis exhibited that BNPs inhibit AChE noncompetitively, Km remain constant, whereas Vmax decreased from 7.24 to 6.1 (18.23% to 31.11%). Ag/Au BNPs do not affect KIapp, while increasing Vmaxiapp from 11.07 to 16 (17.39% to 67.4%). The Km, Ki, and KI were described to be 0.024 mM, 169 μg, and 91 μg, respectively. So, it is concluded that small‐size, stable, potent Ag/Au BNPs were synthesized from HH extract, and consequently, these BNPs significantly inhibit AChE activity in rat brain homogenate and thus prove to be a significant remedy in the treatment of AD.