Abstract
Porcine VIP was synthesized from three segments. The segments, VIP(1–6), VIP(7–13), and VIP(14–28), were synthesized via the Repetitive Excess Mixed Anhydride (REMA) method. The low solubility of the C-terminal segment was greatly improved by a temporary substitution of Asn 28 by a β-t-butyl aspartic acid ester. The segments VIP(1–6) and VIP(7–13) were purified by HPLC and coupled via the mixed anhydride method. The product was purified by gel filtration. VIP was synthesized from VIP(1–13) and VIP(14–28) by the same procedure. After deprotection, Met 17-sulfoxide reduction, and purification by ion-exchange chromatography, the product was found to have the expected amino acid composition and biological potency. A HPLC purified sample was compared with several commercial preparations of varying purity.
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