Abstract
Abstract Two fluorescent nucleosides were synthesized via incorporating benzothiophene and benzofuran into position 5 of 6-aza-uridines. With more intensive push-pull interactions between electron-deficient uridine core and electron-rich extended aromatic moieties, both extended 6-aza-uridines show enhanced bathochromic shifts with remarkable sensitivity to polarity, pH or viscosity changes, suitable as a useful nucleobase probe to be sensitive to changes of microenvironment. The extended nucleoside modified by benzofuran display much high emission quantum yields and emission intensity in apolar solvents.
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