Abstract

An increasingly crucial therapeutic issue revolves around treating bacterial infections in chronic skin wounds that have developed resistance to antibiotics. Despite the current advancements in diagnosis, a significant number of patients still experience therapeutic failure of anti-microbials or antibiotic treatments. In this study, sodium titanate nanotubes (Na-TiNTs) were synthesized via a straightforward hydrothermal approach to facilitate the immobilization of Gentamicin (Gn) and Phenytoin (PhT). The samples were extensively characterized using different techniques, including XRD, FTIR, SEM/EDX, TEM, TGA, and BET. The result reveals that synthesized Na-TiNTs are highly porous rods in shape, with an internal diameter of 5 nm and an outer diameter ranging from 10 to 15 nm. After 16 h of encapsulation, Gn had an encapsulation efficiency (EE%) of 80 ± 2.3 %, while PhT had 89.0 ± 3.1 %. Also, Gn and PhT were released linearly, reaching 95.3 ± 1.6 % and 97.3 ± 1.6 % after 96 h. The highest doses of Gn/Na-TiNTs and PhT/Na-TiNTs, about 500 mg/mL, reduced the cell viability value from 98.2 ± 2.2 % for the untreated cell to 85.1 ± 1.6 % and 88.3 ± 3.4 %, respectively. In addition, PhT/Na-TiNTs ZOI was 28.1 ± 2.3 and 30.1 ± 1.7 mm, andthe ZOI of Gn/Na-TiNTs was 39.0 ± 1.6 mm 45.0 ± 2.4 mm for S. aureus and E. coli, indicating that PhT had antibacterial potency comparable to the standard antibiotic but less than Gn/Na-TiNTs. In vitro wound closure of approximately 87 % for Gn/Na-TiNTs and exhibited a 95 % for PhT/Na-TiNTs after 72 h. The initial findings indicate that Gn/Na-TiNTs and PhT/Na-TiNTs exhibit a sustained drug release profile, which holds potential as a viable strategy for enhancing the antibacterial efficacy and promotewound healing.

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