Abstract
This paper describes the synthesis of active ester copolymers from N-isopropylacrylamide (NIP) with N-acryloxysuccinimide (NAS), aminolysis of active esters, and determination of their average molecular weights. As a model, thermolysin was attached to the polymer to form soluble-insoluble immobilized enzymes capable of being centrifuged above their Tc, because poly(N-isopropylacylamide) (poly(NIP)) has a lower critical solution temperature (Tc) of about 31–33°C. The effects of immobilization of enzymes have been studied including the amount of active ester, time, pH, temperature, and ratio of polymer to enzyme. The tests of thermal stability and repeated precipitation and separation showed that the use of Tc as the technique of separation is possible.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.