Abstract
Two new 3'-phosphorylation procedures of oligoribonucleotide have been worked out. By means of using donor with a 3'-terminal phosphate group, under low ratio of donor/acceptor (1:1.1 to 1:2), nonadecanucleotide AUUCCGGACUCGUCCACCAp, which corresponds to the 3'-terrninal nucleotides 58～76 of yeast alanine tRNA, has been synthesized in good yields (87～90%) through ligation by T_4 RNA ligase of four component fragments: AUUC, CGGA, CUCGUCCA and CCAp.
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