Abstract
Most of diabetes patients have type 2 diabetes mellitus or non insulin dependent diabetes mellitus. Treatment of type 2 diabetes mellitus can be conducted by inhibiting the α-glucosidase enzyme which converts carbohydrates into glucose. Sulochrin is one of the potential compounds which can inhibit the function of α-glucosidase enzyme. This study was carried out to obtain data of sulochrin binding with α-glucosidase enzyme as α-glucosidase inhibitor using Radioligand Binding Assay (RBA) method. The primary reagent required in RBA is the labeled radioactive ligand ( radioligand). In this study, the radioligand was sulochrin- 125 I. Prior to the sulochrin- 125 I synthesis, sulochrin-I was synthesized. Sulochrin-I and sulochrin- 125 I were synthesized and their bindings were studied using RBA. The molecular formula of the synthesized Sulochrin - I is C 17 H 15 O 7 I and its molecular weight is 457.9940 . The s ulochrin - 125 I was synthesized from sulochrin -I by isotope exchange method. From RBA, the dissociation constant (K d ) and the maximum binding (B max ) were found as 26.316 nM and 9.302 nM, respectively. The low K d indicates that sulochrin is capable of binding to α-glucosidase. Received: 08 November 2013; Revised: 28 February 2014; Accepted: 28 March 2014
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