Synthesis of sulfated dendrimers and studies of their anticoagulant and antiinflammatory activity

Abstract

Three hyperbranched dendrimers (polyglycidols) and the corresponding sulfated derivatives, differing in the average molecular weight, were synthesized. The compounds obtained were characterized in detail by mass spectrometry and 1H and 13C NMR spectroscopy, enabling estimation of the sizes of the corresponding molecules. Assignment of signals and identification of key structural blocks were performed using 2D homo- and heteronuclear spectroscopy (COSY, HSQC). The spectra of the sulfated derivatives showed the absence of the signals for the glycerol moiety with the free OH groups, that confirms exhaustive sulfation. The studies of antiinflammatory and anticoagulant activities of the polyanionic samples showed that all the compounds manifest weak antiinflammatory activity, however, their anticoagulant activity displayed in preliminary trials seems to be considerable. The results obtained indicate that it is reasonable to study in more detail biological activity of sulfated dendrimers of this type in terms of their anticoagulant properties.