Abstract
Methyl esters of abscisic acid (ABA) analogues and metabolites dissolved in 0.1–1.0 M sodium methoxide in MeO 2H replaced four of their sidechain 1H atoms with 2H (C-4 and C-6) over a period of 4 to 8 weeks. The esters were hydrolysed by adding 2H 2O to provide 4H 4 labelled free acids. ABA, 1′,2′-epoxy-β-ionylidene acetic and phaseic acids have been labelled in this way. The method can be used to make 3H labelled materials.
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