Synthesis of spin labelled 2,4-dinitrophenols and their activities in the uncoupling of oxidative phosphorylation in rat liver mitochondria

Abstract

Two spin labelled dinitrophenols [1-Hydroxyl-5-N-(1-oxyl-2,2,5,5-tetramethyl-3-amino-pyrrolidinyl)-2,4-dinitrobenzene, and 1-Hydroxyl-5-N-(1-oxyl-2,2,5,5-tetramethyl-3-amino-methylpyrrolidinyl)-2,4-dinitrobenzene] were prepared and found to retain full activity in the uncoupling of oxidative phosphorylation in rat liver mitochondria. Spin labelled 2,4-dinitrobenzene analogues are inactive.