Abstract
Bacteria that are depleted of intracellular thymidine nucleotide pools incorporate [ 3H]thymine at full specific activity, allowing the detection of early intermediates in DNA replication. A short pulse of [ 3H]thymine is incorporated almost exclusively into very small DNA chains which, during further incorporation of thymine, are converted into larger chains and high molecular weight DNA. The synthesis of these small DNA chains depends on the products of dna genes B, E and G. Analysis of the DNA by gel filtration on Sepharose 2B revealed an abundance of extremely short DNA chains while the frequency of larger chains decreased exponentially with increasing size. This size distribution of small DNA chains suggests a mechanism of DNA replication in which larger chains (Okazaki pieces, Okazaki et al., 1968 a) arise through joining of extremely short polynucleotide chains in a process resembling crystallization rather than unidirectional chain elongation.
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