Synthesis of silver nanoparticles using bioreductors from clove leaf extract (Syzygium aromaticum) and test of its antibacterial activity

Abstract

Synthesis of silver nanoparticles using clove leaf extract (Syzygium aromaticum) bioreductor and its antibacterial activity has been studied. Silver nanoparticles were synthesized using the green synthesis method with clove leaf extract (Syzygium aromaticum) as a bioreductor. Nanoparticles were analyzed and characterized by a spectrophotometer by UV-Vis, FTIR, SEM, XRD, and antibacterial activity. Antibacterial activity testing was carried out against Pseudomonas Aeruginosa and Bacillus Subtilis using the disc paper method. Silver nanoparticles were successfully synthesized by a reduction method using clove leaf extract (Syzygium aromaticum). The resulting nanoparticles have varying wavelengths in the range 420-435 nm and have non-uniform shapes with the resulting particle crystal size diameter based on the Debye-Scherer formula is 36.57 nm. FT-IR spectrum comparison of clove leaf extract and silver nanoparticles shows the contribution of the OH functional group in reducing silver ions which is indicated by its presence decrease in absorbance intensity in the area of wave number 3400 cm−1 after the formation of nanoparticles silver. Antibacterial activity of silver nanoparticles against Pseudomonas Aeruginosa and Bacillus Subtilis which showed that the inhibition zone diameter was 11 mm and 9 mm.