Synthesis of silver nanoparticles by Lactobaciluus acidophilus 01 strain and evaluation of its in vitro genomic DNA toxicity

S Karthick Raja Namasivayam,E Kumar Gnanendra,R Reepika

doi:10.1007/bf03353635

S Karthick Raja Namasivayam, E Kumar Gnanendra + Show 1 more

Open Access

https://doi.org/10.1007/bf03353635

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Abstract

Abstract Silver nanoparticles synthesized by dried biomass of Lactobaciluus acidophilus 01 strain was evaluated against toxicity of genomic DNA isolated from bacteria (E. coli) fungi (Beauveria bassiana) algae (Seenedesmus acutus) and human blood adopting standard condition was discussed in the present study. Synthesized silver nanoparticles were characterized by UV-Vis spectroscopy and SEM. The UV-Vis spectroscopy revealed the formation of silver nanoparticles by yielding the typical silver plasmon absorption maxima at 430 nm and SEM micrograph indicates the uniform spherical particles within the size range of 45∼60 nm. The energy dispersive X-ray spectroscopy (EDX) of the nanoparticle confirmed the presence of elemental silver signal as strong peak. The above synthesized silver nanoparticles didn’t cause any toxic effect on all the tested genomic DNA at all tested concentrations which reveals nil genomic nanoparticles induced toxicity.

Highlights

Nanotechnology is significant on account of its pre-eminence upon the comprehension, use, and control of matter at magnitudes of a minute scale, akin to approaching atomic levels, with which to manufacture new substances, instruments, and frameworks [1]
The flask was incubated at 30qC for 32 h on a rotary shaker at 200 rpm, and the cells were harvested by centrifugation at 3000 rpm for 15 minutes and the collected cell pellets was dried in hot air oven at 60qC for 24 h
In a typical procedure of nanoparticle synthesis, the dried cell pellets were washed thrice with Milli-Q-deinosied water and the washed cell pellets were transferred to 500 ml of conical flask containing 200 ml of milli-Q-deinosied water and kept on a rotary shaker at 200 rpm for 72 h

Summary

Introduction

Nanotechnology is significant on account of its pre-eminence upon the comprehension, use, and control of matter at magnitudes of a minute scale, akin to approaching atomic levels, with which to manufacture new substances, instruments, and frameworks [1]. Production of silver nanoparticles can be achieved through different methods. Some chemical methods cannot avoid the use of toxic chemicals in the synthesis protocol.

Results

Conclusion