Synthesis of proteins in cells infected with herpesvirus: V. Viral glycoproteins

Abstract

The glycoproteins synthesized by pseudorabies virus-infected rabbit kidney cells have been analyzed. Two types of glycoproteins have been distinguished: (1) glycoproteins that are present primarily in the cytoplasmic fraction of the infected cells and that do not become part of the virions—these glycoproteins are excreted into the culture fluids in relatively large amounts; (2) glycoproteins that are found associated with the nuclei of infected cells—these glycoproteins have an electrophoretic profile similar to that of the glycoproteins associated with viral particles and probably become part of the viral envelope. Nuclear DNA-containing viral particles and mature viral particles possess glyco-proteins with identical electrophoretic profiles; however, the nuclear particles contain fewer of these proteins. These results indicate that the DNA-containing viral particles isolated from the nuclei have the same membrane proteins as mature virus but that these proteins are present in nuclear particles in lesser amounts than in mature particles.