Abstract
Waste frying oil is abundant and can be used as a cheaper carbon source in PHA production. The utilization of waste frying oil for production of PHA not only enhances the economics of such products,but also provides with a strategy to overcome disposal problems. In this study, the waste frying oil is used as the carbon source for the synthesis of PHA by Cupriavidus necator (formerly known as Ralstonia eutropha) to find out the best growth conditions in order to provides the basis for producing PHA in industry. The results showed that the optimal conditions for production of PHA was at the temperature of 25°C, pH 7, C/N ratio (w/w) 20:0.75 and incubation time 72h. The maximum concentration of PHA was obtained as 7.04g/L.
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