Abstract
Magnetic resonance imaging (MRI) is one of best imaging technologies to monitor transplanted stem cells, because of its high anatomical resolution and safety. In this study, we aim to synthesize Poly (styrene sulphonate) coated Gd3+ - Poly (lactide co glycolic acid) Perfluorooctyl Bromide nanoparticles (PSS-coated Gd3+@NPs) for stem cell labeling using both 1H and 19F MRI. At a weight ratio 20 of PLGA/ Gd3+, NPs have a spherical shape with the hydrodynamic size at 180 nm with zeta potential at -53 mV. In vitro, we obtain the intended T1 relaxation acceleration of water on 1H MRI. The NPs have low toxicity to the human mesenchymal stem cells (hMSC) up to 1 mg/ml. They could internalize into the stem cells via caveolae-mediated endocytosis efficiently, confirmed by flow cytometry analysis, and gives a good contrast in both channels 1H and 18F MRI scans. In conclusion, our NPs have shown a great potential as a dual-contrast agent in MRI for stem cell monitoring.
Highlights
Rising incidence of degenerative diseases such as osteoarthritis and neurological disorders has pressed the demand for regenerative therapies in countries with aging population
Stem cells are labeled with contrast agents such as super paramagnetic iron oxides nanoparticles (SPION), Gadolinium containing compounds, or fluorinated nanoparticles in order to be detected using Magnetic resonance imaging (MRI) [1-4]
Based on our previous studies, we found that coating poly(styrene sodium sulphonate) onto poly(lactide -co-glycolic acid) perfluorooctyl bromide nanoparticles (PSS-coated NPs) would be used to label human mesenchymal stem cells for tracking using 19F MRI [13,14]
Summary
Rising incidence of degenerative diseases such as osteoarthritis and neurological disorders has pressed the demand for regenerative therapies in countries with aging population. Stem cells are labeled with contrast agents such as super paramagnetic iron oxides nanoparticles (SPION), Gadolinium containing compounds, or fluorinated nanoparticles in order to be detected using MRI [1-4]. Among these three contrast agents, SPION is mostly used for its high sensitivity in 1H MRI and its ability to create a negative contrast (darkness) in the images. Fluorinated compounds, usually called tracers rather than contrast agents, provide high nuclear magnetic sensitivity [8-10] without exerting interference with the intrinsic signal sources
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