Abstract
Introduction: In the current study we designed a sophisticated drug delivery nanoparticle to control the methylprednisolone succinate delivery rate and affect the cancer cell growth in culture condition. Materials and methods: Magnetic nanoparticles were synthesized through co-precipitation method. Fe3O4 was first prepared via co-precipitation method and then its surface was functionalized with polyamidoamine (PAMAM) nanodendrimer. PAMAM synthesis trait was detected via FT-IR and SEM methods. Methylprednisolone drug was loaded on PAMAM@Fe3O4 and its effect against cancer cell lines was studied. In order to slow down drug release rate from nanoparticles, PAMAM@Fe3O4 were coated with trimethylchitosan (TMC) after drug loading. Performance of PAMAM@Fe3O4@TMC nanoparticles loaded with mmethylprednisolone, were evaluated against two cell lines to detect the cytotoxic and apoptotic effects by invert light scanning microscopy, immunoassay, and LDH cytotoxicity Kit. Results: According to SEM, image size of Fe3O4 was 4.79-6.37nm, which is smaller than nanodendrimer (6.30-43.67 nm). FT-IR spectrum for ester bond Methylacrylate @ Ethylendiamin was obtained to be 1720-1730 cm-1. FT-IR Spectrums 600 cm-1, 1000 cm-1 belong to Fe3O4, and Fe3O4@ NH2. Also, trimethyl chitosan coated Nanoparticle @ Drug, smearing trimethyl chitosan with Glutaraldehyde, created cross link between TMC monomer at low drug doses in each complete nanoparticle, gave confidence drug side effect, therefore, this nanoparticle could be safe for future cancer therapy. Conclusion: The results showed that drug delivery via PAMAM@Fe3O4 nanoparticle reduces cell viability in vitro condition.
Highlights
In the current study we designed a sophisticated drug delivery nanoparticle to control the methylprednisolone succinate delivery rate and affect the cancer cell growth in culture condition
Fe3o4 was 5.61-17.46 nm, which was smaller than nanodendrimer (6.30-43.67 nm)
Cells were exposed to different treatment media, cell viability was measured by lactate dehydrogenase (LDH) assay after a 24h-exposure
Summary
In the current study we designed a sophisticated drug delivery nanoparticle to control the methylprednisolone succinate delivery rate and affect the cancer cell growth in culture condition. Methylprednisolone drug was loaded on PAMAM@Fe3O4 and its effect against cancer cell lines was studied. Performance of PAMAM@Fe3O4@TMC nanoparticles loaded with mmethylprednisolone, were evaluated against two cell lines to detect the cytotoxic and apoptotic effects by invert light scanning microscopy, immunoassay, and LDH cytotoxicity Kit. Results: According to SEM, image size of Fe3O4 was 4.79-6.37nm, which is smaller than nanodendrimer (6.30-43.67 nm). Dendrimer is known because of its unique three molecular structure, and branch like arms built high number of active terminal end groups [2] These terminal end groups help dendrimers to bond to the surface of nanoparticles sake direct transfer to target. Nowadays scientists have more challenges on target therapy [3] This procedure without any damage to other normal cells has goal preference to old drug using form [4]. The localization filed of magnetic nanoparticle has a power to make assignable spot methylprednisolone deliverance
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