Abstract
The purpose of this study was to characterize the synthesis of the plasminogen activator by synchronized normal and Rous sarcoma virus-transformed chick embryo fibroblasts in culture. The plasminogen activator activity of the cell lysates was assayed on 125I-fibrin-coated Petri dishes and was expressed as the radioactivity released from the plates. It was found that normal cells only produce detectable levels of plasminogen activator in the S-phase and late G2-phase or mitosis of the mitotic cycle. In contrast, transformed cultures synthesize this activator throughout the entire cell cycle although its rate of synthesis fluctuates and reaches a maximum in the G2-M periods. We have also found that the appearance of plasminogen activator activity following fibroblasts infection with Rous sarcoma virus depends on both the synthesis of cellular DNA and cell division.
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