Synthesis of Oligosaccharides by Growing Culture of Leuconostoc Mesenteroides

Abstract

With all glucobioses (eleven types) as acceptors, Leuconostoc mesenteroides (NRRL B-512) was grown on a sucrose medium. The trisaccharides produced were analyzed for their yields, and the trisaccharide structures were determined after separation on a column. The yield of the tri-and higher-saccharides indicated that isomaltose (28%) was the most efficient acceptor and maltose (24%) was next. With the other eight glucobioses, oligosaccharides were obtained in 3_??_15% yield. Among these sugars, α, β-trehalose (15%) and β, β-trehalose (11%) were efficient acceptors next to maltose, but α, α-trehalose was inert. In every case, except for cellobiose, α-glucosyl transfer occurred to the position 6 of non-reducing moiety of glucobiose. The sugars produced contained five new trisaccharides which were isolated as pure compounds.