Synthesis of Oligodeoxynucleotides Containing 2-Substituted Guanine Derivatives Using 2-Fluoro-2′-Deoxyinosine as Common Nucleoside Precursor

Abstract

Oligonucleotides containing 2-substituted guanine derivatives with double-helix stabilizing molecules such as spermine, spermidine and propylimidazole have been prepared using protected 2-fluoro-2′-deoxyinosine phosphoramidite and two different protective strategies: the p-nitrophenylethyl (NPE) and the t-butylphenoxyacetyl groups. Melting studies show a large increase on the melting temperatures of duplexes containing these 2-substituted guanine derivatives.