Synthesis of novel phosphoramidite derivatives bearing pyrenyl and dansyl groups

Abstract

The synthesis of novel phosphoramidites is reported starting with the readily available 4-(1-pyrenyl)-butanol or 5-(dimethylamino)naphthalene-1-sulfonic acid chloride, respectively. Both amidites can be used on the DNA synthesizer to prepare 5′ - or internally labeled oligonucleotides without an acyl amide linkage to the fluorescent dye.