Abstract
Two new fluorinated fluorescent dyes, 6,8-difluoro-7-hydroxy-4-methylcoumarin (Marina Blue TM) and 3-carboxy-6,8-difluoro-7-hydroxycoumarin (Pacific Blue TM), exhibit excellent photophysical properties among a series of novel fluorinated 7-hydroxycoumarins. Most of these fluorinated coumarins have quantum yields (0.63 to 0.89) equal to or higher than that of the parent compound (0.63), which, in combination with their lower p K as and higher photostability, make them superior fluorescent dyes for use as reporter molecules in biological systems.
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